Aspergillus organism has the ability to produce a wide spectrum of infections including Mycotoxicosis, allergic manifestations, localized non-invasive infection in normal host and invasive infection in the compromised host. Aspergillus fumigatus is the most common species associated with human diseases followed by Aspergillus flavus. Lung Aspergillosis includes Primary pulmonary aspergillosis, Acute invasive pulmonary disease, Aspergillus pseudotuberculosis, Chronic necrotizing pulmonary aspergillosis and Aspergilloma. All positive results should be confirmed by microbiologic and clinical evaluation. False positive results are commonly seen in children and in patients on antimicrobial therapy like Piperacillin -Tazobactum and Amoxicillin-Clavulanate.