Anti HBs appears after HBsAg disappears and persists thereafter. It is rarely detected in the presence of HBsAg in patients with Acute Hepatitis B, but 10-20% of patients with Chronic Hepatitis B may show low levels of Anti HBs. Presence of Anti HBs has been shown to be important in protection against HBV infection. Passively acquired antibody to HBV as in the case of blood transfusion and recent immunoglobulin therapy does not signify immunity.