Primary post natal Rubella virus infection is typically a mild self limiting disease but in utero infection may severely damage the fetus. In case of acute primary infection, IgM has been detected 4-15 days after the appearance of rash. The levels begin to decline after 36-70 days but infrequently may be detected upto 180 days. In suspected cases of primary infection, the optimum time of specimen collection is 1-2 weeks after the onset of rash.